Objective molecular criteria for oocyte quality and developmental potential: a proteomic study in inbred mice

ABSTRACT: Current understanding of oocyte quality and developmental potential maintains that stochastic or epigenetic processes modulate the action of determinants that are laid in the oocyte during oogenesis. These determinants are considered to be rather conserved across mice, leading different strains of mice to be used interchangeably. We challenged this assumption and studied the relationship between oocyte composition and developmental quality in four inbred strains of mice, namely 129Sv, C57Bl/6, C3H/HeN and DBA/2J. These oocytes showed large variability developmental competence and embryo quality irrespective of the developmental stimulus (fertilization, somatic cell nuclear transfer, parthenogenesis). To unravel the molecular basis of the observed phenotypes we applied state-of-the-art prot

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Michele Boiani 

PROVIDER: E-MTAB-5013 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress