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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Saccharomyces cerevisiae transcriptome analysis of conditional yjr141w-psd mutant compared to control strain

ABSTRACT: The experiment investigates the consequences of Yjr141w downregulation on gene expression in yeast. The essential protein Yjr141w was depleted from cells using a tagged variant (Yjr141w-psd). The photo-sensitive degron (psd) module initiates degradation of the tag and its associated protein upon blue-light exposure. Altogether, we compared samples of the control yeast strain (ESM356-1) grown in darkness (3 arrays) and grown exposed to blue-light (wavelength 465 nm; light-flux 30 µmol m-2 s-1) with the Yjr141w-psd strain (YAL81) grown in darkness and grown exposed to blue-light.

INSTRUMENT(S): Affymetrix GeneChip Scanner 3000 7G

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Christof Taxis

PROVIDER: E-MTAB-5022 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Proteasome Activity Is Influenced by the HECT_2 Protein Ipa1 in Budding Yeast.

Lutz Anne P AP Schladebeck Sarah S Renicke Christian C Spadaccini Roberta R Mösch Hans-Ulrich HU Taxis Christof C

Genetics 20180308 1

The ubiquitin-proteasome system (UPS) controls cellular functions by maintenance of a functional proteome and degradation of key regulatory proteins. Central to the UPS is the proteasome that adjusts the abundance of numerous proteins, thereby safeguarding their activity or initiating regulatory events. Here, we demonstrate that the essential <i>Saccharomyces cerevisiae</i> protein Yjr141w/Ipa1 (Important for cleavage and PolyAdenylation) belongs to the HECT_2 (homologous to E6-AP carboxyl termi ...[more]

PMID: 29519818

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