RNA-seq transcriptome profiling of the psychrophilic phytoplankton Fragilariopsis cylindrus under environmentally relevant growth limitations

ABSTRACT: We performed RNA-sequencing experiments to examine the differential regulation of genes in the genome of the Southern Ocean diatom Fragilariopsis cylindrus including diverged alleles. RNA-seq was performed on three replicate samples for each experimental condition. Phytoplankton cells were grown under six different experimental conditions including (1) optimal growth, (2) freezing temperatures, (3) elevated temperature, (4) elevated carbon dioxide concentrations, (5) low iron concentrations and (6) prolonged darkness. Total RNA was extracted using a guanidinium thiocyanate-phenol-chloroform extraction protocol, followed by DNase I treatment and RNA purification (Quiagen). First strand cDNA synthesis was performed using random hexamers. Library preparation was performed using the RNA-seq Sa

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Fragilariopsis cylindrus

SUBMITTER: Thomas Mock 

PROVIDER: E-MTAB-5024 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress