Overexpression of Integrin α11 Induces Cardiac Fibrosis in Mice

ABSTRACT: The integrin α11-overexpressing transgenic mouse strain was generated to further understand the function of α11, after generation of our integrin α11-KO strain. Analysis of the strain showed that integrin α11 was highly expressed in heart, which correlated with a fibrotic heart phenotype. To understand the molecular mechanisms underlying this phenotype, we then performed Microarray to investigate the differentially expressed genes between the WT and TG mice (α11-overexpressing mice) which may result from integrin α11 overexpression. The microarray was performed on RNA-isolates from the heart apex of 1-year-old WT (n=4) and α11-TG (n=6) mice. Total RNA isolation was performed using RNeasy Fibrous Tissue Mini Kit (Qiagen) in accordance with the manufacturer’s protocol. Quality of RN

ORGANISM(S): Mus musculus

SUBMITTER: Rita Holdhus 

PROVIDER: E-MTAB-5040 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress