Modulation of the bulk cell density as decisive parameter and simple method for mesendodermal patterning of human pluripotent stem cells

ABSTRACT: 1 million human pluripotent cells cultured as suspension aggregates (hES3-NKX2.5 cell line) were treated with 0, 7.5 or 15µM CHIR99021 in 1 or 3 ml RPMI/B27 (w/o insulin) for 24h to induce the differentiation of human pluripotent stem cells (hPSCs). CHIR99021 is a potent GSK3 inhibitor that results in prominent WNT pathway activation and thereby induces mesendodermal differentiation.

ORGANISM(S): Homo sapiens

SUBMITTER: Henning Kempf 

PROVIDER: E-MTAB-5051 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress