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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Radiation exposure of human coronary artery endothelial cells - 1 day, 7 days, 14 days post-irradiation

ABSTRACT: In recent years there is a growing epidemiological indication of excess risk of cardiovascular disease at low doses of ionizing radiation without a clear-cut threshold. It is proposed that damage to the vascular endothelium is critical in radiation-related cardiovascular diseases. In order to identify and better understand the underlying molecular mechanisms of ionizing radiation on endothelial cells, we performed a microarray analysis on immortalized human coronary artery endothelial cells irradiated with different doses (0.00, 0.05, 0.10, 0.50 and 2.0 Gy). RNA was extracted at different time points after irradiation (1 day, 7 days, 14 days).

ORGANISM(S): Homo sapiens

SUBMITTER: An Aerts

PROVIDER: E-MTAB-5054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Fe ions and X-ray radiation exposure of Est2-immortalised human coronary artery endothelial cells - 1 day and 7 days post-irradiation

Project description:In recent years there is a growing epidemiological indication of excess risk of cardiovascular disease at low doses of ionizing radiation without a clear-cut threshold. It is proposed that damage to the vascular endothelium is critical in radiation-related cardiovascular diseases. In order to identify and better understand the underlying molecular mechanisms of high LET (Fe ions) and low LET (X-ray) radiation on endothelial cells, we performed a microarray analysis on immortalized human coronary artery endothelial cells irradiated with 2.00 Gy and compared them with sham-irradiated samples. RNA was extracted at different time points after irradiation (1 day, 7 days).

2017-10-02 | E-MTAB-5754 | biostudies-arrayexpress

Radiation exposure of mouse embryonic brain - 2 h, 24 h post-irradiation

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2015-07-01 | E-MTAB-2720 | biostudies-arrayexpress

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2015-04-01 | E-MTAB-2632 | biostudies-arrayexpress

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AM_GeneExpressionAfterIonisingRadiation

Project description:Abstract Purpose: There is a recognised need to develop new methods of high throughput, rapid and minimally invasive individual dose assessment for radiation exposure. The aim of this work is to establish a panel of highly radiation responsive genes suitable for biological dosimetry and to explore inter-individual variation in response to ionising radiation exposure.Materials and method: Analysis of gene expression in response to radiation was carried out using three independent techniques (microarray, Multiplex Quantitative RT-PCR and nCounter Analysis System) in human lymphocytes in culture and circulating blood exposed ex vivo from the same donors. Results: Variations in transcriptional response to exposure to ionising radiation analysed by microarray allowed the identification of genes which can be validated and measured accurately as biomarkers of radiation exposure using other techniques. We have identified genes which are consistently up-regulated following exposure at different time points to either 2 or 4 Gy of X-rays, for all individuals in blood and cultured lymphocytes. Most down-regulated genes including cyclins, centromeric and mitotic checkpoints proteins, particularly those associated with chromosome instability and cancer can only be detected in dividing cells. Conclusions: The data provides evidence that there are a number of genes which seem suitable for biological dosimetry, like SESN1, GADD45A, CDKN1A, CCNG1, FDXR, BBC3 and MDM2. ÊThese biomarkers could potentially be used for triage after large-scale radiological incidents. Variations in transcriptional response accurately measured by MQRT-PCRæ may allow the identification of biomarkers of radiation sensitivity and individual susceptibility and therefore being useful in radiation oncology.

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2012-11-28 | E-GEOD-42547 | biostudies-arrayexpress

Transcription profiling by array of human embryonic stem cell line H9 in response to ionizing radiation.

Project description:The study aimed to elucidate the whole human genome wide changes in gene expression in cultured human embryonic stem cells after ionizing radiation exposures

2010-12-01 | E-MEXP-2596 | biostudies-arrayexpress

Expression analysis of the first-generation offspring of irradiated male mice

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2012-12-17 | E-GEOD-42621 | biostudies-arrayexpress

Comparative gene-expression analysis on the first-generation offspring of irradiated male and female mice

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2013-01-29 | E-GEOD-42933 | biostudies-arrayexpress

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