Transcriptional profiling of the tuberculin skin test in healthy volunteers and in rheumatoid arthritis patients treated with methotrexate or anti-TNF therapy

ABSTRACT: Anti-TNF therapy has transformed the management of rheumatoid arthritis (RA); however little is known about its effects on cell mediated immune responses and TNF-inducible activity at the site of inflammation. Here, we used the tuberculin skin test (TST) as a standardised in vivo human challenge model to make systems level assessments of the effects of anti-TNF therapy in a prototypic cell mediated immune response. RA patients (treated with monoclonal anti-TNF antibodies (adalimumab or infliximab), the soluble TNF receptor etanercept or the standard therapy methotrexate) and healthy volunteers with immunological memory to Mycobacterium tuberculosis antigens were identified using an interferon-γ release assay of peripheral blood. Two units tuberculin or an equivalent volume of saline was i

ORGANISM(S): Homo sapiens

SUBMITTER: Carolin Turner 

PROVIDER: E-MTAB-5093 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress