Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analysis of the effect of ppGpp, cdiAMP, and cdiGMP on Saccharomyces cerevisiae


ABSTRACT: Apart from the induction of host immune responses, any influence mediated by the presence of bacterial signalling molecules inside host cells on the latter’s physiology remains poorly defined. To determine the effects of the presence of ppGpp, cdiAMP and cdiGMP in the cytosol on the functioning of a simple eukaryotic cell, we have heterologously expressed enzymes for their synthesis in the budding yeast Saccharomyces cerevisiae. Transcriptome analysis was employed to measure any effects on gene transcription following induction of synthetase expression.

INSTRUMENT(S): Affymetrix Genechip scanner 3000, Affymetrix Genechip hybridisation oven 650

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Andrew Hesketh 

PROVIDER: E-MTAB-5128 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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