Medicago truncatula strain:Jemalong Transcriptome or Gene expression

ABSTRACT: An intron-spliced hairpin RNA approach was used for the targeted silencing of the MtTdp1α gene encoding the αisoform of tyrosyl-DNA phosphodiesterase 1 in Medicago truncatula Gaertn. Tyrosyl-DNA phosphodiesterase 1, involved in the repair of DNA topoisomerase I-mediated DNA damage, has been poorly investigated in plants. RNA-Seq analysis, carried out in the MtTdp1α-depleted plants, revealed different levels of transcriptional modulation (up- and down-regulation, alternative splicing, activation of alternative promoter) in genes involved in DNA damage sensing, DNA repair, and chromatin remodelling.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Medicago truncatula

SUBMITTER: Alma Balestrazzi 

PROVIDER: E-MTAB-5141 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress