RNA-seq transcriptome profiling of the psychrophilic diatom Fragilariopsis cylindrus under six treatments simulating conditions within sea ice

ABSTRACT: We performed RNA-sequencing experiments to examine gene expression in the genome of the cold-adapted (psychrophilic) marine diatom Fragilariopsis cylindrus to six different treatments simulating conditions found within sea ice. RNA-seq was performed on replicate samples for each experimental condition. Phytoplankton cells were grown under six different experimental conditions including (I) 0℃ and salinity 34 (seawater before ice formation), (II) two days at -4℃ and salinity 34 (early freezing or frazil ice formation), (III) eight days at -4℃ and salinity 34 (late freezing, frazil ice layer formation), (IV) two days at -4℃ and salinity 52 (initialization of brine channel formation in ice), (V) eight days at -4℃ and salinity 52 (established brine channel formation and young consolidated colu

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Fragilariopsis cylindrus

SUBMITTER: Graham Underwood 

PROVIDER: E-MTAB-5153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress