Deep Mutational Scanning of HIV tat and rev in a non-overlapped context

ABSTRACT: This dataset provides allele counts and raw fastqs for deep mutational scanning of the HIV-1 genes tat and rev when not-overlapped with one another (placed in the nef locus) as described in Fernandes et al. Functional segregation of overlapping genes in HIV Cell 2016 (in revision). Preselection (input) and post selection (replicate 1/2) files for every possible point mutant of these two HIV proteins from the NL4-3 background are given.Tab delimited files including codon counts across the amplicons are also included and are probably the most useful thing to most researchers. The data here was used to generate Figures 3 and 4 and 7 and might be of general use for people interested in deep mutational scanning, looking for signatures of epistasis in rev or tat, or reanalyzing and mining the

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): HIV-1 vector pNL4-3

SUBMITTER: Jason Fernandes 

PROVIDER: E-MTAB-5154 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress