Transition of embryonic stem cells from ground state pluripotency

ABSTRACT: Mouse embryonic stem cells were maintained in minimal and chemically-defined culture conditions supporting naive pluripotency. Inhibitors of the Gsk3 (CHIR99021) and Mek/Erk (PD0325901) pathways were withdrawn, cultures maintained for 24 hours, and subsequently sorted by flow cytometry based on fluorescence of a short-half-life Rex1(Zfp42)::GFP reporter into two populations: Rex1-high cells, functionally capable of reversion to naive pluripotency, and Rex1-low cells that have exited the naive state.

ORGANISM(S): Mus musculus

SUBMITTER: Paul Bertone 

PROVIDER: E-MTAB-5304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress