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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Profiling Period 2 (PER2) mediated pathways following ischemic preconditioning (IPC) of the mouse heart using a genome array with 352 abundantly expressed and well-characterized micro RNAs

ABSTRACT: Ischemic preconditioning (4 cycles of 5 min ischemia and 5 min reperfusion) with a final reperfusion time of 120 minutes was performed in C57BL/6N (The Jackson Laboratory) or Per2-/- mice. Heart tissue was snap-frozen with clamps pre-cooled to the temperature of liquid nitrogen. Micro RNA was isolated with Trizol (Invitrogen) and purified using RT2 qPCR-Grade miRNA Isolation Kit (SABiosciences-Qiagen). cDNA template was generated using RT2 miRNA First Strand Kit (SABiosciences-Qiagen). miRNA expression was performed using RT2 miRNA PCR Array Mouse miFinder (SABiosciences-Qiagen).

ORGANISM(S): Mus musculus

SUBMITTER: Tobias Eckle

PROVIDER: E-MTAB-5401 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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