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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Microarray analyses of C. albicans sak1 deletion mutant in complex liquid medium (YPD)

ABSTRACT: Sak1 (orf19.3840) of Candida albicans was found to be a kinase which phosphorylates and thereby activates Snf1, a highly conserved regulator of nutrient stress responses. Accordingly, a sak1 deletion mutant failed to grow on many alternative (i.e., non-glucose) carbon sources, but also showed a filamentation defect upon growth under glucose limitation and exhibited reduced virulence. To better define the effects of Sak1 on C. albicans metabolic adaptations, these transcriptional analyses were performed in complex medium, using a sak1 deletion mutant in comparison to the wild type.

INSTRUMENT(S): GenePix 4200AL

ORGANISM(S): Candida albicans

SUBMITTER: Stefanie Allert

PROVIDER: E-MTAB-5415 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The Snf1-activating kinase Sak1 is a key regulator of metabolic adaptation and in vivo fitness of Candida albicans.

Ramírez-Zavala Bernardo B Mottola Austin A Haubenreißer Julia J Schneider Sabrina S Allert Stefanie S Brunke Sascha S Ohlsen Knut K Hube Bernhard B Morschhäuser Joachim J

Molecular microbiology 20170418 6

The metabolic flexibility of the opportunistic fungal pathogen Candida albicans is important for colonisation and infection of different host niches. Complex regulatory networks, in which protein kinases play central roles, link metabolism and other virulence-associated traits, such as filamentous growth and stress resistance, and thereby control commensalism and pathogenicity. By screening a protein kinase deletion mutant library that was generated in the present work using an improved SAT1 fli ...[more]

PMID: 28337802

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