Translational profiling of HEK293 cells exposed to cold shock (32 degrees C) and untreated (37 degrees C)

ABSTRACT: This experiment investigates the effect of mild cold-shock (32 degrees C, 24 hours) on the translatome of human HEK293 cells. After cold-shock, cells were treated with cycloheximide to block elongation, then sucrose density gradients were used to separate post-nuclear extracts into polysome and subpolysome fractions. The pooled polysomal fraction was labelled with Cy5, the pooled subpolysomal fraction was labelled with Cy3, and both were hybridised to the same array.

ORGANISM(S): Homo sapiens

SUBMITTER: Amandine Bastide 

PROVIDER: E-MTAB-5437 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress