FunGenES: UED-LF2: HOXB4 can enhance the differentiation of embryonic stem cells by modulating the haematopoietic niche

ABSTRACT: Haematopoietic differentiation of embryonic stem (ES) cells in vitro has been used as a model to study early haematopoietic development and it is well documented that haematopoietic differentiation can be enhanced by over-expression of HOXB4. HOXB4 is expressed in haematopoietic progenitor cells (HPC) where it promotes self-renewal, but it is also expressed in the primitive streak of the gastrulating embryo. This led us to hypothesise that HOXB4 might modulate gene expression in pre- haematopoietic mesoderm and that this property might contribute to its pro-haematopoietic effect in differentiating ES cells. To test our hypothesis we developed a conditionally activated HOXB4 expression system using the mutant oestrogen receptor (ERT2) and showed that a pulse of HOXB4 prior to HPC emergence

ORGANISM(S): Mus musculus

SUBMITTER: Oliver Hummel 

PROVIDER: E-MTAB-547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress