Microarray comparison of NK cell from healthy donors and day 14 post stem cell transplantation

ABSTRACT: NK cells were first isolated from PBMCs using EasySep™ Human NK cell enrichment kit (STEMCELL biotechnology). The magnetically isolated NK cells were stained with anti-CD3 FITC, anti-CD56 Pe-Cy7 and LIVE/DEAD® red stain for a further enrichment, whereby the CD3 negative and CD56 positive live cells were sorted with MoFlo™ cell sorter (Beckman Coulter). The sorted NK cell populations were sent to AROS Applied Biotechnology A/S (Aarhus N, Denmark) to be analysed using GeneChip® Human Transcriptome Array 2.0 (Affymetrix, USA). The transcription profile of NK cells from day 14 post stem cell transplantation was compared with those from healthy donors.

ORGANISM(S): Homo sapiens

SUBMITTER: Jianmin Zuo 

PROVIDER: E-MTAB-5474 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress