Lineage-dependent spatial and functional organisation of the mammalian enteric nervous system

ABSTRACT: Single-cell transcriptomic analysis to delineate key regulators of neurogenic commitment in the enteric nervous system. To resolve the molecular profiles of ENCCs and identify transcriptional signatures of lineage restriction and differentiation, we analyzed their transcriptomes by single-cell RNA-sequencing (scRNA-seq). E12.0 Sox10::CreERT2;Rosa26tdTomato (SER93|tdT) embryos were exposed to a single dose of TM (100g/g of dam body weight) and tdT+ gut cells were isolated 20-24 hours later by flow cytometry.

INSTRUMENT(S): Linux Beowulf cluster, Linux computer, Illumina HiSeq 4000, FACSAria Fusion

ORGANISM(S): Mus musculus

SUBMITTER: Reena Lasrado 

PROVIDER: E-MTAB-5553 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress