A MILI-independent piRNA biogenesis pathway empowers partial germline reprogramming.

ABSTRACT: In mice, the PIWI-piRNA pathway is essential to re-establish transposon silencing during male germline reprogramming. The cytoplasmic PIWI protein MILI mediates piRNA-guided transposon RNA cleavage as well as piRNA amplification. MIWI2-bound piRNAs and its nuclear localization are proposed to be dependent upon MILI function. Here, we demonstrate the existence of a piRNA biogenesis pathway that in the absence of MILI that sustains partial MIWI2 function and reprogramming activity.

INSTRUMENT(S): Illumina HiSeq 1000

ORGANISM(S): Mus musculus

SUBMITTER: Lina Vasilauskaite 

PROVIDER: E-MTAB-5561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress