MicroArray analysis of miRNA expression of human esophageal cell samples and breast cell samples for comparison with multiplexed ligation-based miRNA detection assay

ABSTRACT: This experiment was carried out as part of a benchmarking study for Ligo-miR EZ, a ligation based multiplex miRNA detection assay. Differential analysis was performed for miRNA expression among the cell samples tested with the microarray and Ligo-miR EZ assay to confirm that Ligo-miR EZ gave the same results as an established, commercially available array. Primary, normal, non-immortalized human esophageal epithelial cells (HEEPIC), along with esophageal cancer cell lines (SKGT4 and OE33), were purchased from ScienCell Research Laboratories (Carlsbad, California, USA) and Sigma Chemical (St Louis, Missouri, USA), respectively. The Barrett’s esophageal cell lines (CHTRT and QHTRT) were gifts of Dr. Peter Rabinovitch, Fred Hutchinson Cancer Center. HEEPIC and CHTRT, QHTRT cells were cultur

ORGANISM(S): Homo sapiens

SUBMITTER: John Kilburn 

PROVIDER: E-MTAB-5575 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress