Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

The Sinorhizobium meliloti NfeR1-dependent transcriptome probed with Sm14kOLI microarrays

ABSTRACT: NfeR1 is a S. meliloti sRNA highly expressed in response to osmostress and during the symbiotic interaction of this bacterium with alfalfa plants. We have probed the NfeR1-dependent transcriptome by competitive hybridization on Sm14kOLIGO microarrays of RNA from the wild-type strain Sm2B3001 and from a mutant derivative lacking NfeR1 upon growth in unmodified minimal medium or after 1 h shock with 400 mM NaCl.

ORGANISM(S): Sinorhizobium meliloti

SUBMITTER: JosÃ© JimÃ©nez-Zurdo

PROVIDER: E-MTAB-5588 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A conserved α-proteobacterial small RNA contributes to osmoadaptation and symbiotic efficiency of rhizobia on legume roots.

Robledo Marta M Peregrina Alexandra A Millán Vicenta V García-Tomsig Natalia I NI Torres-Quesada Omar O Mateos Pedro F PF Becker Anke A Jiménez-Zurdo José I JI

Environmental microbiology 20170511 7

Small non-coding RNAs (sRNAs) are expected to have pivotal roles in the adaptive responses underlying symbiosis of nitrogen-fixing rhizobia with legumes. Here, we provide primary insights into the function and activity mechanism of the Sinorhizobium meliloti trans-sRNA NfeR1 (Nodule Formation Efficiency RNA). Northern blot probing and transcription tracking with fluorescent promoter-reporter fusions unveiled high nfeR1 expression in response to salt stress and throughout the symbiotic interactio ...[more]

PMID: 28401641

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Project description:Malic enzymes decarboxylate the tricarboxylic acid (TCA) cycle intermediate malate to the glycolytic end-product pyruvate and are well positioned to regulate metabolic flux in central carbon metabolism. The bacterium Sinorhizobium meliloti has a NAD(P)-malic enzyme (DME) and a NADP-malic enzyme (TME) and DME is required for symbiotic N2-fixation. To help understand the role of these enzymes, we examined growth, metabolic and transcriptional consequences resulting from the deletion of these enzymes. Few effects were observed upon growth with glucose, whereas growth with the gluconeogenic substrate, succinate, resulted in transcriptional and metabolic effects particularly in the dme mutant strains. When grown with succinate, DME mutant cells accumulated hexose sugar phosphates and trehalose, while TME mutants accumulated putrescine. Succinate-grown DME mutant cells also showed increased transcription of genes for gluconeogenesis and for pathways such as amino acid and fatty acid synthesis that divert metabolites away from the TCA cycle. These data suggested that, DME is required to regulate the levels of TCA cycle intermediates and that the activity of TME is insufficient to prevent the accumulation of TCA cycle intermediates in cells utilizing succinate as carbon source. Consistent with this, in short-1-3 hour incubations with succinate, dme mutant cells excreted large amounts of malate whereas little malate was excreted from tme or wild-type cells. These results support the suggestion that DME is required for N2-fixation in alfalfa because it is required for synthesis of pyruvate and acetyl-CoA and the rapid metabolism of C4-dicarboxylates supplied by the plant. RNA expression was measured for S. meliloti in exponential phase grown in MOPS (morpholinpropanesulfonic acid) buffered minimal media under 2 growth conditions: 1) 15 mM succinate, 2) 15 mM glucose; using wild type cells as well as dme and tme mutant strains (6 experiments, 2 replicates: total 12 samples)

Dataset Information

The Sinorhizobium meliloti NfeR1-dependent transcriptome probed with Sm14kOLI microarrays

Publications

A conserved α-proteobacterial small RNA contributes to osmoadaptation and symbiotic efficiency of rhizobia on legume roots.

Similar Datasets

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