Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of wheat after inoculation with an adapted and a non-adapted Magnaporthe isolate or mock-inoculation to identify candidate genes for nonhost resistance against blast disease


ABSTRACT: In this experiment the transcriptome reprogramming in wheat during host and nonhost interaction with Magnaporthe sp. was analyzed in a time-series approach. Seven days old wheat plants of cv. Renan were mock-inoculated or inoculated with adapted Magnaporthe isolate Br116.5 from wheat or non-adapted isolate CD180 from Pennisetum. After 6, 12, 24 and 48 hours the abaxial epidermis was sampled. Total RNA was extracted using the RNeasy Plant Mini Kit with on-column DNase digestion (Qiagen, Hilden, Germany), and hybridized to Agilent 44k oligonucleotide arrays.

INSTRUMENT(S): Agilent G2505B scanner

ORGANISM(S): Triticum aestivum

SUBMITTER: Rhoda Delventhal 

PROVIDER: E-MTAB-5635 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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