RNA-seq of chemically reset human naive pluripotent stem cells

ABSTRACT: A simple method is presented to reset human pluripotent cells to a naive state via transient histone deacetylase inhibition and maintenance in chemically-defined naive stem cell culture media. Cells can be reset without transgenes and expanded continuously either on feeders or alternative substrates in feeder-free conditions. Multiple cell lines of varying origin were reset and characterised in parallel with conventionally cultured counterparts.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Homo sapiens

SUBMITTER: Paul Bertone 

PROVIDER: E-MTAB-5674 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress