ICLIP analysis of RNA binding sites of Stau2 protein in mouse brain cells

ABSTRACT: Stau2 iCLIP of mouse brain was performed to identify RNA binding sites of Stau2 protein in mouse brain cells. The experiment was performed in triplicate, and each of the replicates was split into two separate halves at the cDNA stage, which together led to 6 separate datasets. The iCLIP protocol includes the following steps; Embryonic day 18 whole mouse brain was dissociated and irradiated with UV-C light, and the cells were then lysed using a buffer containing detergents. The RNAs were partially digested, and Stau2 and the cross-linked RNA fragments were immunoprecipitated using anti-Stau2 antibody. A DNA adaptor was then ligated to the RNA fragments and the cross-linked RNAs were further purified by SDS-PAGE and nitrocellulose membrane transfer. The RNAs were extracted from the membrane

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Mus musculus

SUBMITTER: Yoichiro Sugimoto 

PROVIDER: E-MTAB-5703 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress