Genome-wide screen for differential DNA methylation associated with neural cell differentiation in mouse

ABSTRACT: Cellular differentiation involves widespread epigenetic reprogramming, including modulation of DNA methylation patterns. Using Differential Methylation Hybridization (DMH) in combination with a custom DMH array containing more than 53,000 features covering more than 16,000 murine genes, we carried out a genome-wide screen for cell- and tissue-specific differentially methylated regions (tDMRs) in undifferentiated embryonic stem cells (ESCs), in in-vitro induced neural stem cells (NSCs) and 8 differentiated embryonic and adult tissues. Unsupervised clustering of the generated data showed distinct cell- and tissue-specific DNA methylation profiles, revealing 202 significant tDMRs (p<0.005) between ESCs and NSCs and a further 380 tDMRs (p<0.05) between NSCs/ESCs and embryonic brain tissue. We

ORGANISM(S): Mus musculus

SUBMITTER: Reinhold Wasserkort 

PROVIDER: E-MTAB-576 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress