Beijing Institute of Radiation Medicine Enterovirus Protein Array

ABSTRACT: Enterovirus 71 (EV71) and coxsackievirus A16 (CA16) are major causative agents for hand, foot and mouth disease (HFMD). In China, more than 70,756 children were infected and 40 died from the disease in recent years. This study aimed to develop a protein chip that can simultaneously detect and differentiate the antibodies induced by EV71 and CA16 simultaneously. The structure protein vp1 and vp3 from the two viruses were purified and spotted onto an aldehyde groupmodified glass slide at 1mg/ml. After that, the protein chip was reacted with the corresponding positive serum against these viruses, hybridized with a Cy3-labeled secondary antibody and scanned using the popular GenePix 4000B Microarray Scanner. In this study, Both IgG and IgM serum antibody to EV71 and CA16 were detected using p

ORGANISM(S): Homo sapiens

DISEASE(S): IgG negative for EV71 and CA21

SUBMITTER: Shengqi Wang 

PROVIDER: E-MTAB-577 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress