Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression in cells encapsulated within different types of alginate microcapsules

ABSTRACT: D1 multipotent mesenchymal stromal cells (D1‐MSCs,ATCC® CRL 12424TM) and genetically modified with the lentiviral vector pSIN‐EF2‐Epo‐Pur to express erythropoietin (EPO). D1‐MSCs genetically modified to release EPO were immobilized into 3D alginatepoly‐ L‐lysine‐alginate (APA) microcapsules. Since different formulations of alginate 1.5%, poly‐L‐lysine 0.05%, alginate 0.1% and washings were designed, two types of APA microcapsules were obtained: Biological microcapsules (made of Biological formulations) and Technological microcapsules (made of Technological formulations). The expression of the cells under different conditions of these two types of microcapsules have been analyzed. The present analysis proved that the mechanical properties of the matrix in which cells are enclosed influences drastically gene expression

ORGANISM(S): Mus musculus

SUBMITTER: Rosa Hernandez 

PROVIDER: E-MTAB-5786 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The role of osmolarity adjusting agents in the regulation of encapsulated cell behavior to provide a safer and more predictable delivery of therapeutics.

Gonzalez-Pujana Ainhoa A   Rementeria Aitor A   Blanco Francisco Javier FJ   Igartua Manoli M   Igartua Manoli M   Pedraz Jose Luis JL   Santos-Vizcaino Edorta E   Hernandez Rosa Maria RM  

Drug delivery 20171101 1

Transplantation of cells within alginate microspheres has been extensively studied for sustained drug delivery. However, the lack of control over cell behavior represents a major concern regarding the efficacy and the safety of the therapy. Here, we demonstrated that when formulating the biosystem, an adequate selection of osmolarity adjusting agents significantly contributes to the regulation of cell responses. Our data showed that these agents interact in the capsule formation process, influen  ...[more]

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