The effect of bacterial capsule on macrophage transcriptional responses to Streptococcus Pneumoniae.

ABSTRACT: Streptococcus Pneumoniae capsule is a major virulence factor. The capsule is known to inhibit complement fixation and phagocytes, but its impact on innate immune responses is not known. To address this question, we compared transcriptional responses by human monocyte derived macrophages in responses to wild type S. pneumoniae with isogenic mutant bacteria lacking their capsule. We conducted these experiments with and without macrophage pretreatment with cytochalasin D to evaluate the impact of phagocytosis, and we also made comparisons to mutant S.pneumoniae lacking bacterial lipoprotein which is known to be necessary for maximal innate immune responses.

ORGANISM(S): Homo sapiens

SUBMITTER: Mahdad Noursadeghi 

PROVIDER: E-MTAB-5894 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress