The plasmid-borne Type II Secretion System of Escherichia coli O157:H7 isolate Sakai mediates adherence to spinach. Experiment 1: screening E coli O157:H7 Sakai genes for adherence to spinach roots.

ABSTRACT: Food-borne illness arising for Shiga-toxigenic Escherichia coli is often linked to consumption of fruit and vegetables as the bacteria have the ability to interact with plants and use them as alternative or secondary hosts. Attachment of the bacteria to host tissue is one of the first steps in the interaction, and, as with mammalian hosts, has shown to be mediated by a combination of non-specific and specific adhesin-mediated interactions. We took a high-throughput positive-selection approach to investigate adherence mechanisms for E. coli O157:H7 isolate Sakai by inoculating a BAC clone library onto spinach, which was quantified by microarray hybridisation and gene loci enrichment measured using a Bayesian hierarchical model. The screen involved four successive rounds of adherence to s

INSTRUMENT(S): Agilent G2505B scanner

ORGANISM(S): Escherichia coli

SUBMITTER: Pete Hedley 

PROVIDER: E-MTAB-5923 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress