Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptome profiling of knock mutant and RNAi lines for small GTPase, nucleolar GTP-binding protein 1 (NOG1) in Arabidopsis

ABSTRACT: Gene expression profiling of knock out mutant NOG1-1 and RNAi line NOG1-2 was studied in comparison to wild type in leaves of seven days old plants.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Yuhong Tang

PROVIDER: E-MTAB-5946 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

The small GTPase, nucleolar GTP-binding protein 1 (NOG1), has a novel role in plant innate immunity.

Lee Seonghee S Senthil-Kumar Muthappa M Kang Miyoung M Rojas Clemencia M CM Tang Yuhong Y Oh Sunhee S Choudhury Swarup Roy SR Lee Hee-Kyung HK Ishiga Yasuhiro Y Allen Randy D RD Pandey Sona S Mysore Kirankumar S KS

Scientific reports 20170823 1

Plant defense responses at stomata and apoplast are the most important early events during plant-bacteria interactions. The key components for the signaling of stomatal defense and nonhost resistance have not been fully characterized. Here we report the newly identified small GTPase, Nucleolar GTP-binding protein 1 (NOG1), functions for plant immunity against bacterial pathogens. Virus-induced gene silencing of NOG1 compromised nonhost resistance in N. benthamiana and tomato. Comparative genomic ...[more]

PMID: 28835689

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Project description:Comparative analysis of tobacco leaves transcriptomes unveils carotenoid pathway potentially determined the characteristics of aroma compounds in different environmental regions. Tobacco (Nicotiana tabacum) is a sensitive crop to environmental changes, and a tobacco with unique volatile aroma fractions always formed in specific ecological conditions. In order to investigate the differential expressed genes caused by environmental changes and reveal the formation mechanism of characteristics of tobacco in three different aroma tobacco regions of Guizhou Province, Agilent tobacco microarray was adapted for transcriptome comparison of tobacco leaves in medium aroma tobacco region Kaiyang and light aroma tobacco regions Weining and Tianzhu. Results showed that there was big difference among the gene expression profiles of tobacco leaves in different environmental conditions. A total of 517 differential expressed genes (DEGs) between Weining and Tianzhu were identified, while 733 and 1,005 genes differentially expressed between Longgang and another two tobacco regions Weining and Tianzhu, respectively. Compared with Longgang, up-regulated genes in Weining and Tianzhu were likely involved in secondary metabolism pathways, especially carotenoid pathway, including PHYTOENE SYNTHASE, PHYTOENE DEHYDROGENASE, LYCOPENE ε-CYCLASE, CAROTENOID β-HYDROXYLASE and CAROTENOID CLEAVAGE DIOXYGENASE 1 genes, while most down-regulated genes played important roles in response to temperature and light radiation, such as heat shock proteins. Gene Ontology and MapMan analyses demonstrated that the DEGs among different environmental regions were significantly enriched in light reaction of photosystem II, response of stimulus and secondary metabolism, suggesting they played crucial roles in environmental adaptation and accumulation of aroma compounds in tobacco plants. Through comprehensive transcriptome comparison, we not only identified several stress response genes in tobacco leaves from different environmental regions but also highlighted the importance of carotenoid pathway genes for characteristics of aroma compounds in specific growing regions. Our study primarily laid the foundation for further understanding the molecular mechanism of environmental adaptation of tobacco plants and molecular regulation of aroma substances in tobacco leaves. In order to investigate the differential expressed genes caused by environmental changes and reveal the formation mechanism of characteristics of tobacco in three different aroma tobacco regions of Guizhou Province, Agilent tobacco microarray was adapted for transcriptome comparison of tobacco leaves in medium aroma tobacco region Kaiyang and light aroma tobacco regions Weining and Tianzhu.

Dataset Information

Transcriptome profiling of knock mutant and RNAi lines for small GTPase, nucleolar GTP-binding protein 1 (NOG1) in Arabidopsis

Publications

The small GTPase, nucleolar GTP-binding protein 1 (NOG1), has a novel role in plant innate immunity.

Similar Datasets

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