Sequence Requirements for Cascade Function in a Type I-E CRISPR System: Plasmid Loss

ABSTRACT: We generated a collection of 13 plasmids, with each plasmid containing a variant of a CRISPR protospacer targeted by spacer 8 of the E. coli CRISPR-I array. We transformed the plasmids as a pool into delta cas3 E. coli cells expressing all other cas genes constitutively. We then transformed these cells with either an empty vector or a plasmid expressing the Cas3 nuclease. DNA surrounding the protospacers was PCR-amplified and sequenced.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Anne Stringer 

PROVIDER: E-MTAB-5969 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress