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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Sequence Requirements for Cascade Function in a Type I-E CRISPR System: ChIP Library-seq

ABSTRACT: We generated a collection of 13 plasmids, with each plasmid containing a variant of a CRISPR protospacer targeted by spacer 8 of the E. coli CRISPR-I array. We transformed the plasmids as a pool into delta cas3 E. coli cells expressing all other cas genes constitutively, with FLAG-tagged casA. We then used ChIP to enrich for CasA-bound protospacers. DNA surrounding the protospacers was PCR-amplified from input (pre-immunocrecipitation) and ChIP (post-immunoprecipitation) samples and sequenced.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

SUBMITTER: Anne Stringer

PROVIDER: E-MTAB-5972 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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