Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

16 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tissue-specific DNase-seq during Drosophila melanogaster embryogenesis

ABSTRACT: Chromatin accessibility mapping by DNase-seq on FACS-isolated cell populations during Drosophila melanogaster embryogenesis (6-8 hrs after egg-laying)

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot

PROVIDER: E-MTAB-5999 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Tissue-specific DNase-seq Time Course during Drosophila melanogaster embryogenesis

Project description:Chromatin accessibility mapping by DNase-seq on whole embryo and FACS-isolated cell populations during Drosophila melanogaster embryogenesis at 2-4 hrs, 4-6 hrs, 6-8 hrs, 8-10 hrs and 10-12 hrs after egg-laying. Note that the two 8 bases long UMIs clipped from read1 and read2 are present in the FastQ file header (followed by the 8 bp long invariant sample barcode)

2020-10-30 | E-MTAB-8881 | biostudies-arrayexpress

Drosophila melanogaster 4C-Seq experiments in whole embro and mesoderm cells during embryogenesis

Project description:Chromosome conformation capture (4C-Seq) in Drosophila Twist-H2B embryos (carrying nuclear tag specifically in the mesoderm) during embryogenesis was performed, anchoring on 107 different viewpoints. Two timepoints (3-4hrs and 6-8hrs after egg laying) and two tissue context (whole embryo and mesoderm) were assayed. Two independent collections were performed at each timepoint.

2014-06-26 | E-MTAB-2180 | biostudies-arrayexpress

Hi-C in the Drosophila wild-type embryos at 2-4h after egg-laying

Project description:Hi-C was performed on 2 biological replicates of wild-type Drosophila embryos at 2-4 hrs after egg-laying. Each library was first checked on MiSeq and then deeply sequenced on HiSeq 2000 (4 lanes for each biological replicate)

2023-05-14 | E-MTAB-12070 | biostudies-arrayexpress

Hi-C in the Drosophila wild-type embryos at 6-8 h and 16-18 hrs after egg-laying

Project description:Hi-C was performed on wild-type Drosophila melanogaster embryos at 6-8 hrs and 16-18 hrs after egg-laying, on 2 biological replicates per time point. Each library was first checked on MiSeq and then deeply sequenced on HiSeq 2000 (4 lanes for each biological replicate). This study completes the E-MTAB-12070 Hi-C dataset on 2-4 hrs wild-type Drosophila melanogaster embryos. Both studies use the same experimental procedures (protocols, sequencer model and run parameters)

2024-01-24 | E-MTAB-13267 | biostudies-arrayexpress

Zfh1 ChIP-seq in Drosophila melanogaster embryos at 4-6h and 6-8h AEL

Project description:Canton S flies (as wild type) were bred at 25C and tightly staged 2-hr embryos were collected after 2 times of 2-hr prelay. The embryos were aged on the apple juice plates and aged to 4-6 and 6-8 hr. For each time point, two independent Chromatin immunoprecipitation (ChIP) experiments were performed using two different antibodies and compared to two independent mock ChIPs using the corresponding pre-immune serum. One Zfh1 antibody was generated in Guinea pig against full-length Zfh1 in the Furlong laboratory, and the other was a generous gift from Dr. Ruth Lehmann. ChIPs were optimized using the enrichment of a binding site identified in a pilot experiment of Zfh1- ChIP-on-chip measured by real-time PCR as previously described. The quality of each IP was also assessed by real-time PCR. Solexa libraries were prepared according to the manufacturer's recommendations. Library quality was assessed on a 2100 Bioanalyzer system (Agilent). All samples were single-end sequenced with 36-bp reads using an Illumina Genome Analyzer IIX by the EMBL Genomics Core Facility.

2019-07-01 | E-MTAB-7422 | biostudies-arrayexpress

Capture-C in D. melanogaster carrying second (CyO) and third (TM3) balancer chromosomes

Project description:We used a Drosophila melanogaster line (a "double balancer") carrying balancer chromosomes for both the second (CyO) and third (TM3) chromosomes, and crossed it to an isogenic wild-type "virginizer" line. Trans-heterozygous adults from the F1 generation were further crossed to the wild-type parental line to obtain the pool of N1 embryos. Allele-specific chromosome conformation capture (Capture-C) was used to measure changes in chromatin organization on both chromosomes.

2019-04-24 | E-MTAB-7513 | biostudies-arrayexpress

Hi-C in D. melanogaster carrying second (CyO) and third (TM3) balancer chromosomes

Project description:We used a Drosophila melanogaster line (a "double balancer") carrying balancer chromosomes for both the second (CyO) and third (TM3) chromosomes, and crossed it to an isogenic wild-type "virginizer" line. Trans-heterozygous adults from the F1 generation were further crossed to the wild-type parental line to obtain the pool of N1 embryos. Allele-specific chromosome conformation capture (Hi-C) was used to measure changes in chromatin organization on both chromosomes.

2019-04-24 | E-MTAB-7512 | biostudies-arrayexpress

Time course DNase assays on Drosophila melanogaster and Drosophila virilis embryos during embryogenesis

Project description:DNase-seq over 3 matching developmental time points in Drosophila melanogaster and Drosophila virilis embryos was performed. The aim is to assess conservation of hypersensitive regions between two distantly related species. Samples were sequenced using Illumina HiSeq.

2019-10-19 | E-MTAB-3797 | biostudies-arrayexpress

Time course DNase assays on Drosophila melanogaster and Drosophila virilis embryos during embryogenesis II

Project description:DNase-seq over 2 matching developmental time points in Drosophila melanogaster and Drosophila virilis embryos was performed. The aim is to assess conservation of hypersensitive regions between two distantly related species. Samples were sequenced using Illumina NextSeq. This Study is an extension to the previously published Study E-MTAB-3797.

2021-05-21 | E-MTAB-9480 | biostudies-arrayexpress

Time course Pho/dSfmbt Chromatin Immunoprecipitation on Drosophila melanogaster embryos during embryogenesis

Project description:Here we improved BiTS-ChIP (Bonn et al, Nature Protocols 7, 978-994 (2012)) to identify active enhancer and promoter elements genome wide in the 104 cardiomyocytes that constitute the Drosophila heart tube and represents only ~0.5% of the total cell content of the embryo. A transgenic Drosophila strain expressing nuclear GFP under the control of a cardiac specific enhancer (TinC*>GFP) was used for staged embryo collections at stages 13-14 (10-13h of development). After embryo fixation and dissociation, intact fixed nuclei were fluorescent labelling.Â Â Purification of this rare nuclear population was achieved by a two-step sorting procedure, yielding ~98% purity. Chromatin was extracted and used for immunoprecipitation and sequencing (ChIP-seq) to analyze chromatin modifications at promoters (H3K4me3 and H3K27ac) and enhancers (H3K27ac).Â Â Two independent biological replicates (from FACS sorting, chromatin preparations and ChIP-Seq) were performed for each mark and sequenced using Illumina HiSeq.

2017-04-11 | E-MTAB-4585 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data