Time line RNAseq of iPSC-derived neurons from ASD and control subjects.

ABSTRACT: To comprehensively profile early neurodevelopmental alterations in individuals with ASD, we harnessed a time series approach to monitor patient-derived induced pluripotent stem cells (iPSCs) throughout the recapitulation of cortical development. This dataset consists of patient derived neurons that go through all consecutive developmental stages (NSC-derived neurons) as well as a comparative set of iPSC-iNs (neurons generated from the same patients that bypass early NSC-like stages using an Ngn2-transgene approach). For this, we first used fluorescence-activated cell sorting (FACS) to purify a homogeneous population of NSCs based on the expression of the cell-surface markers CD184+/CD271-/CD44-/CD24-/CD15+. To trace ASD and control neurons over time, we performed a series of retroviral lin

INSTRUMENT(S): Illumina HiSeq 2500, TruSeq Stranded mRNA Sample Prep Kit (Illumina), BD InFlux Cytometer (Becton-Dickinson), Cutadapt, STAR

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Schafer 

PROVIDER: E-MTAB-6018 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress