Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

21 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNA array profiling on human pluripotent stem cell-derived cardiomyocytes exposed to doxorubicin

ABSTRACT: Cardiomyocytes derived from human pluripotent stem cells were exposed to the cardiotoxic drug Doxorubicin in order to assess the utility of this cell system as a model for drug-induced cardiotoxicity. Cells are exposed to different concentrations of doxorubicin for up to 48 hours followed by a 12 days recovery period.

ORGANISM(S): Homo sapiens

SUBMITTER: Jane Synnergren

PROVIDER: E-MTAB-6052 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

MicroRNAs as potential biomarkers for doxorubicin-induced cardiotoxicity.

Holmgren Gustav G Synnergren Jane J Andersson Christian X CX Lindahl Anders A Sartipy Peter P

Toxicology in vitro : an international journal published in association with BIBRA 20160406

Anthracyclines, such as doxorubicin, are well-established, highly efficient anti-neoplastic drugs used for treatment of a variety of cancers, including solid tumors, leukemia, lymphomas, and breast cancer. The successful use of doxorubicin has, however, been hampered by severe cardiotoxic side-effects. In order to prevent or reverse negative side-effects of doxorubicin, it is important to find early biomarkers of heart injury and drug-induced cardiotoxicity. The high stability under extreme cond ...[more]

PMID: 27033315

Similar Datasets

Cohort 2: miRNA expression profiling in the whole blood of Primary Sjogren's Syndome patients

Project description:Whole blood total RNA was isolated form PaxGene tubes using the PaxGene total RNA extraction kits. miRNA expression was profiled in Primary Sjogren's Syndrome patients and controls in order to independently validate differentially expressed miRNAs between patients and controls and between patient subgroups that were identified in 'Cohort 1'.

2022-04-01 | E-MTAB-5505 | biostudies-arrayexpress

Cohort 3: miRNA expression profiling in the whole blood of Primary Sjogren's Syndome patients

2022-04-01 | E-MTAB-5506 | biostudies-arrayexpress

Cohort 1: miRNA expression profiling in the whole blood of Primary Sjogren's Syndome patients

Project description:Whole blood total RNA was isolated form PaxGene tubes using the PaxGene total RNA extraction kits. miRNA expression was profiled in Primary Sjogren's Syndrome patients and controls in order to identify differentially expressed miRNAs between patients and controls and between patient subgroups.

2022-04-01 | E-MTAB-5504 | biostudies-arrayexpress

miRNA profiling of celiac disease

Project description:microRNAs were profiled in healthy controls, classic celiac patients (CD), CD patients with anemia and GFD treated CD with normalization of duodenal mucosa all CD conditions were related to controls. For each group, five patients were pooled. One replicate per experiment

2013-04-19 | E-GEOD-46201 | biostudies-arrayexpress

Identification of Angiotensin II-senstive microRNAs in mouse abdominal aortic endothelium

Project description:Endothelial-enriched total RNAs were obtained from the suprarenal region of the abdominal aorta which is the murine AAA prone area in AngII-infused C57BL/6 mice. At 12 or 36h post-AngII pump implantation, endothelial-enriched RNAs from four abdominal aortas were pooled to obtain ~30 ng total RNA as one array sample, performed in triplicates. All RNA samples used for miRNA microarray study passed the initial quality control test and each sample was linearly amplified.

2014-07-01 | E-GEOD-54943 | biostudies-arrayexpress

miRNA expression profiling of primary melanoma tumors

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-02-01 | E-GEOD-62372 | biostudies-arrayexpress

MicroRNA-138: A Pro-survival OncomiR for Glioma Stem Cells is Associated with Tumor Recurrence [miRNA]

Project description:We report the identification of microRNA-138 (miR-138), as a molecular signature of GSCs and demonstrate a vital role for miR-138 to promote growth and survival of bona fide tumor-initiating cells with self-renewal potential. Total RNA from Glioma Stem Cells and Neural Stem Cells were subjected to microRNA microarray analysis, 3 replicates each.

2012-12-20 | E-GEOD-39486 | biostudies-arrayexpress

miRNA expression profiling of primary melanoma tumors (cohort II)

Project description:We performed global microRNA expression profiling of a cohort of primary melanoma patient samples linked to a well-annotated clinical database. The goal of this study was to identify microRNA that are associated to or correlated with various clinical parameters and patient outcomes. Candidate microRNA were identified for building prognostic models and functional testing. 119 primary melanoma samples were analyzed in two color arrays. The reference sample used was an equal combination of all samples analyzed. By this design, ratio data of test sample/reference is mean-centered data.

2015-02-01 | E-GEOD-62371 | biostudies-arrayexpress

Factors affecting the yield of microRNAs from laser microdissectates of formalin-fixed tissue sections

Project description:Sixth generation Exiqon® locked nucleic acid miRCURY™ LNA microarrays were used to profile in duplicate the expression of microRNAs in 250 or 400 ng of RNA isolated from a laser microdissectate of a formalin-fixed and paraffin-embedded human non-small cell lung cancer tumor. Laser microdissection was performed on a hematoxylin-eosin-stained, 8 um-thick section of formalin-fixed and paraffin-embedded tissue. The microdissectate was treated with proteinase K overnight at 55 ºC. Total RNA from the lysate was then extracted using FFPE RNA Isolation Kit from Norgen Biotek® (Thorold, Canada). RiboGreen assay was used to quantify RNA in the preparation. Two-hundred-fifty or 400 ng of RNA was used in duplicate for dual-channel microarray-based microRNA expression profiling. Sample RNA was labeled with the Cy3-like Hy3™ dye, mixed with a human universal reference RNA (product number AM6000, Ambion®, Austin, TX) labeled with the Cy5-like Hy5™ dye, and hybridized to the two-color miRCURY™ arrays.

2011-09-07 | E-GEOD-31946 | biostudies-arrayexpress

A Pro-survival OncomiR for Glioma Stem Cells is Associated with Tumor Recurrence

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-12-20 | E-GEOD-37366 | biostudies-arrayexpress