Single-cell RNA-seq analysis of LPS- and palmitate-stimulated THP-1 macrophages

ABSTRACT: In this study we performed single-cell transcriptome analysis of THP-1 macrophages, stimulated with high levels of free fatty acids (palmitate, PAL) typical for obese adipose tissue microenvironment or lipopolysaccharide (LPS), representing a classical stimulus activating innate immune response. Analysing full transcriptomes of individual cells, we were able to distinguish 3 macrophage transcriptional states and decipher gene regulatory pathways underlying macrophage state identity in both stimulations.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Maria Metsger 

PROVIDER: E-MTAB-6075 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress