Transcriptional profiling of tfap2a;tfap2c loss of function in zebrafish single whole embryos at 15 somites post fertilisation

ABSTRACT: We have genetically ablated the neural crest by simultaneously knocking out tfap2a and tfap2c in developing zebrafish embryos. We have collected 10 single embryos at the 15 somites stage from all nine possible genotypic combinations which can be found when heterozygous parent carriers of tfap2a and tfap2c are crossed. Embryos were prepped in singular, barcoded and RNA-Seq libraries were created using True-Seq stranded RNA-Seq LT kit. Libraries were paired end sequenced at 75bp on an Illumina Hi-Seq 2500.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Danio rerio

SUBMITTER: Christopher Dooley 

PROVIDER: E-MTAB-6106 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress