Multifaceted Activity of Cytokinin at Distinct Phases of Leaf Development Shapes Its Size and Structure in Arabidopsis thaliana

ABSTRACT: We performed a transcriptome profiling on the leaf 3 microdissected from plants with altered cytokinin levels 3h after activation at 9 DAS, when this leaf is normally fully composed of proliferating cells to get an insight into dynamics of molecular network underlying cell responses to CK excess and deficiency in proliferating cells. Seeds of Arabidopsis thaliana transgenic lines (CaMV35S>GR>ipt and CaMV35S>GR>HvCKX2) and corresponding wild-types (Col-0) were sown on nylon meshes placed on half-strength Murashige and Skoog (MS) medium containing 0.5% (w/v) MES and 0.8% (w/v) agar. After 2 days of stratification, plants were removed to growing chambers with 16-h day/8-h night, 21 C and 19 C, respectively, under continuous light (110 mol m-2 s-1). DEX-inducible lines and wild-types were acti

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Plant Systems Biology data submission 

PROVIDER: E-MTAB-6109 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress