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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of murine CD4 T cells after pentanoate treatment compared to untreated control

ABSTRACT: To analyse the influence of pentanoate on the differentiation of naive CD4 T cells under Th17 inducing conditions, murine CD4 T cells were isolated from spleen and lymphnodes of FIR/TIGER mice (IL-10 (GFP) - Foxp3 (RFP) - Reporter mice). The T cells were kept for 3 days under Th17 inducing conditions either with 4 mM pentanoate or without pentanoate (control).

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Mus musculus

SUBMITTER: Andrea Nist

PROVIDER: E-MTAB-6114 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The short-chain fatty acid pentanoate suppresses autoimmunity by modulating the metabolic-epigenetic crosstalk in lymphocytes.

Luu Maik M Pautz Sabine S Kohl Vanessa V Singh Rajeev R Romero Rossana R Lucas Sébastien S Hofmann Jörg J Raifer Hartmann H Vachharajani Niyati N Carrascosa Lucia Campos LC Lamp Boris B Nist Andrea A Stiewe Thorsten T Shaul Yoav Y Adhikary Till T Zaiss Mario M MM Lauth Matthias M Steinhoff Ulrich U Visekruna Alexander A

Nature communications 20190215 1

Short-chain fatty acids (SCFAs) have immunomodulatory effects, but the underlying mechanisms are not well understood. Here we show that pentanoate, a physiologically abundant SCFA, is a potent regulator of immunometabolism. Pentanoate induces IL-10 production in lymphocytes by reprogramming their metabolic activity towards elevated glucose oxidation. Mechanistically, this reprogramming is mediated by supplying additional pentanoate-originated acetyl-CoA for histone acetyltransferases, and by pen ...[more]

PMID: 30770822

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