Transcriptomic characterization of the human cell cycle in individual unsynchronized cells

ABSTRACT: Here, we profiled transcription across the cell cycle in single human myxoid liposarcoma cells, without prior synchronization. Flourescence-activated cell sorting was used to infer G1, S or G2/M cell cycle stages after staining cells for DNA content. Single-cell RNA-sequencing was performed according to the Smart-seq2 protocol.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Erik Larsson Lekholm 

PROVIDER: E-MTAB-6142 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress