Global transcriptional response of a Bordetella bronchiseptica Bvg- phase-locked mutant (delta_bvgS) after exposure to blood

ABSTRACT: A Bordetella bronchiseptica Bvg- phase-locked mutant (delta_bvgS) was grown in Stainer-Scholte (SS) broth at 37°C with shaking (220 rpm) overnight. Bacteria were subcultured at a starting OD600 of 0.1 into SS broth and grown at 37°C with shaking (220 rpm) up to an OD600 of 1.0. The cultures were pelleted and bacteria were washed twice with PBS buffer and equal aliquots were resuspended in SS broth (control) or in 100% sheep blood and incubated for 1 hour at 37°C.

ORGANISM(S): Bordetella bronchiseptica

SUBMITTER: Eric Harvill 

PROVIDER: E-MTAB-6147 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress