Dataset Information
Validation of a laboratory assay for prediction of relapse in stage I non-small cell lung cancer (NSCLC) using miRNA profiling of formalin-fixed paraffin-embedded (FFPE) tissue specimens.
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Jesper Dahlgaard
PROVIDER: E-MTAB-618 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Analytical variables influencing the performance of a miRNA based laboratory assay for prediction of relapse in stage I non-small cell lung cancer (NSCLC).
BMC research notes 20111019
<h4>Background</h4>Laboratory assays are needed for early stage non-small lung cancer (NSCLC) that can link molecular and clinical heterogeneity to predict relapse after surgical resection. We technically validated two miRNA assays for prediction of relapse in NSCLC. Total RNA from seventy-five formalin-fixed and paraffin-embedded (FFPE) specimens was extracted, labeled and hybridized to Affymetrix miRNA arrays using different RNA input amounts, ATP-mix dilutions, array lots and RNA extraction- ...[more]
