Stabilized biological production by product addiction

ABSTRACT: Populations of engineered metabolite-producing microorganisms are prone to evolutionary production declines during industrial-scale cultivations. In this study, we develop a synthetic product addiction system in E coli that addicts mevalonic acid production cells to mevalonic acid. Through experimentally simuluated long-term fermentation, we investigate how product-addicted organisms remain stable and avoid formation of genetic subpopulations of fit, non-producing cells.

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Escherichia coli K-12

SUBMITTER: Peter Rugbjerg 

PROVIDER: E-MTAB-6182 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress