RNASeq of multiple Mycoplasma species in wild-type condition including: Mycoplasma pneumoniae, Mycoplasma genitalium, Mycoplasma hyopneumoniae, Mycoplasma capricolum, Mycoplasma gallisepticum, Mycoplasma mycoides

ABSTRACT: The discovery of small open reading frames (smORFs) encoding for polypeptides (SEPs; <100aa) highlights that the coding capacity of the genomes has been underestimated. Most ORF-finding algorithms have historically set a minimum threshold length of 100 aa. As consequence, some transcripts encoding for SEPs, were erroneously discarded or classified as non-coding RNAs (ncRNAs). With this experiments we try to experimentally assess the existence and complexity of these small proteins. The experimental design includes: After growing each Mycoplasma for 6h at 37°C, cells were washed twice with PBS and lysed with 700 µl of Qiazol buffer. Then, samples were lysed with 700 µl of Qiazol buffer. RNA extractions were performed by using the miRNeasy mini Kit (Qiagen) following the instructions of th

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mycoplasma gallisepticum str. R(high)

SUBMITTER: María Lluch-Senar 

PROVIDER: E-MTAB-6203 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress