Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA-seq of estrogen (E2) induced changes of mRNA expression in Sus scrofa male and female blastocysts derived from embryonic day 10

ABSTRACT: Environmental estrogens may affect epigenetic programming as early as the period of preimplantation development. Therefore, we analyzed the effects of continuous gestational estradiol-17β (E2) exposure on male and female embryos. A low dose, close to the no-observed effect level (NOEL - 10 µg E2/kg body weight(bw)/d), a high dose (1000 µg E2/kg bw/d) and carrier only, as control group, was fed to sows from insemination until sampling at day 10 of pregnancy, respectively. 36 samples (n = 5-7 per treatment group and sex) were analyzed by high throughput sequencing.In the high dose group, RNA-sequencing of single embryos revealed 982 differentially expressed genes (DEG) in the female but none in the male blastocysts. Moreover, 62 and 3 DEG were found in female and male embryos of the NOEL dose group, respectively. Thus, maternal E2 treatment during early pregnancy affected gene expression of the embryos at day 10, potentially constituting the basis for long-term adverse effects.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Sus scrofa

SUBMITTER: Stefan Bauersachs

PROVIDER: E-MTAB-6263 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Exposure of pregnant sows to low doses of estradiol-17β impacts on the transcriptome of the endometrium and the female preimplantation embryos†.

Flöter Veronika L VL Bauersachs Stefan S Fürst Rainer W RW Krebs Stefan S Blum Helmut H Reichenbach Myriam M Ulbrich Susanne E SE

Biology of reproduction 20190301 3

Maternal exposure to estrogens can induce long-term adverse effects in the offspring. The epigenetic programming may start as early as the period of preimplantation development. We analyzed the effects of gestational estradiol-17β (E2) exposure with two distinct low doses, corresponding to the acceptable daily intake "ADI" and close to the no-observed-effect level "NOEL", and a high dose (0.05, 10, and 1000 μg E2/kg body weight daily, respectively). The E2 doses were orally applied to sows from ...[more]

PMID: 30260370

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Dataset Information

RNA-seq of estrogen (E2) induced changes of mRNA expression in Sus scrofa male and female blastocysts derived from embryonic day 10

Publications

Exposure of pregnant sows to low doses of estradiol-17β impacts on the transcriptome of the endometrium and the female preimplantation embryos†.

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