RNA sequencing of T helper cell type 2, with CRISPR KO

ABSTRACT: T cells were extracted from spleens of Cas9 mice and activated in vitro with CD3/28 on day 0. They were spinoculated with retroviruses containing sgRNA on day 1. On day 2 and forwarded they were treated with puromycin. On day 4, dead cells were removed by low-G spin and the remaining cells measured by a variant of SMART-seq2

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Johan Henriksson 

PROVIDER: E-MTAB-6285 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress