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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Senataxin resolves R-loops forming at DNA double strand breaks to prevent translocations

ABSTRACT: DNA Double Strands Breaks (DSBs) are highly detrimental since they can lead to mutations and chromosomes rearrangements (amplification, deletion, translocation and chromosome loss). Here, we set to assess the role of senataxin, a RNA:DNA helicase involved in the regulation of transcription and the maintenance of genome integrity, at sites of DNA Double Strand Breaks. We performed ChIP-Seq mapping of senataxin before and after damage, genome-wide DNA:RNA hybrids (DRIP) mapping before and after damage. We also performed RNA-Seq and RNA pol II mapping (total and phosphorylated on serine 2 of the CTD) by ChIP-Seq in undamaged cells to discriminate between damage in active versus inactive regions.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Vincent Rocher

PROVIDER: E-MTAB-6318 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A POLD3/BLM dependent pathway handles DSBs in transcribed chromatin upon excessive RNA:DNA hybrids accumulation

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1,25(OH)2D3 time course in THP-1 cells

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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