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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq of BEAS-2B cells treated with low doses of Ag nanoparticles for 6 weeks

ABSTRACT: Human bronchial lung cells (BEAS-2B) were treated for 6 weeks with low doses (1 µg/mL) of Ag nanoparticles (10 nm citrate coated). At the end of the exposure control and treated samples were submitted for RNA-Seq analysis (Hiseq2500). The overall goal of this experiment was to gain an in-depth understanding of the transcriptomic changes induced by low-dose, long-term exposure of human lung cells to Ag nanoparticles and to generate hypotheses related to their mechanisms of toxicity.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Anda Gliga

PROVIDER: E-MTAB-6321 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-sequencing reveals long-term effects of silver nanoparticles on human lung cells.

Gliga Anda R AR Di Bucchianico Sebastiano S Lindvall Jessica J Fadeel Bengt B Karlsson Hanna L HL

Scientific reports 20180427 1

Despite a considerable focus on the adverse effects of silver nanoparticles (AgNPs) in recent years, studies on the potential long-term effects of AgNPs are scarce. The aim of this study was to explore the effects of AgNPs following repeated low-dose, long-term exposure of human bronchial epithelial cells. To this end, the human BEAS-2B cell line was exposed to 1 µg/mL AgNPs (10 nm) for 6 weeks followed by RNA-sequencing (RNA-Seq) as well as genome-wide DNA methylation analysis. The transcriptom ...[more]

PMID: 29703973

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DNA methylation profiling of BEAS-2B cells exposed to low doses of Ag nanoparticles for 6 weeks

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