Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DNA methylation profiling of BEAS-2B cells exposed to low doses of Ag nanoparticles for 6 weeks

ABSTRACT: Human bronchial lung cells (BEAS-2B) were treated for 6 weeks with low doses (1 µg/mL) of Ag nanoparticles (10 nm citrate-coated). At the end of the exposure control and treated samples were submitted for DNA methylation analysis (Illumina 450k). The overall goal of this experiment was to evaluate potential epigenetic changes associated with low-dose, long-term exposure to Ag nanoparticles.

ORGANISM(S): Homo sapiens

SUBMITTER: Anda Gliga

PROVIDER: E-MTAB-6331 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

RNA-sequencing reveals long-term effects of silver nanoparticles on human lung cells.

Gliga Anda R AR Di Bucchianico Sebastiano S Lindvall Jessica J Fadeel Bengt B Karlsson Hanna L HL

Scientific reports 20180427 1

Despite a considerable focus on the adverse effects of silver nanoparticles (AgNPs) in recent years, studies on the potential long-term effects of AgNPs are scarce. The aim of this study was to explore the effects of AgNPs following repeated low-dose, long-term exposure of human bronchial epithelial cells. To this end, the human BEAS-2B cell line was exposed to 1 µg/mL AgNPs (10 nm) for 6 weeks followed by RNA-sequencing (RNA-Seq) as well as genome-wide DNA methylation analysis. The transcriptom ...[more]

PMID: 29703973

Similar Datasets

RNA-Seq of BEAS-2B cells treated with low doses of Ag nanoparticles for 6 weeks

Project description:Human bronchial lung cells (BEAS-2B) were treated for 6 weeks with low doses (1 µg/mL) of Ag nanoparticles (10 nm citrate coated). At the end of the exposure control and treated samples were submitted for RNA-Seq analysis (Hiseq2500). The overall goal of this experiment was to gain an in-depth understanding of the transcriptomic changes induced by low-dose, long-term exposure of human lung cells to Ag nanoparticles and to generate hypotheses related to their mechanisms of toxicity.

2018-04-30 | E-MTAB-6321 | biostudies-arrayexpress

RNA-Seq of BEAS-2B cells treated with low doses of Ni and NiO nanoparticles as well as NiCl2 for 6 weeks

Project description:Human bronchial lung cells (BEAS-2B) were treated for 6 weeks with low doses (0.5 µg/mL) of Ni and NiO nanoparticles and NiCl2. At the end of the exposure control and treated samples were submitted for RNA-Seq analysis (Hiseq2500). The overall goal of this experiment was to gain an in-depth understanding of the transcriptomic changes induced by low-dose, long-term exposure of human lung cells to Ni and NiO nanoparticles as well as to NiCl2 and to generate hypotheses related to their mechanisms of toxicity.

2020-04-19 | E-MTAB-8903 | biostudies-arrayexpress

Transcription profiling of human bronchial epithelial cells to low doses of cigarette smoke condensate

Project description:This SuperSeries is composed of the following subset Series:; GSE14383: Effects of chronic exposure of human bronchial epithelial cells to low doses of cigarette smoke condensate; GSE14385: Response of bronchial epithelial cells to low doses of cigarette smoke condensate and subsequent demethylation agent Experiment Overall Design: Refer to individual Series

2009-08-09 | E-GEOD-14461 | biostudies-arrayexpress

Transcription profiling of human ronchial epithelial cells to low doses of cigarette smoke condensate and subsequent demethylation agent

Project description:The study seeks to identify the epigenetic changes caused by exposure of to cigarette smoke condensate. To this goal human bronchial epithelial cells, BEAS-2B, were treated with 5-aza-2âdeoxycitidine and trychostatin A (5AzaC/TSA) subsequent to a chronic exposure (1 month) to cigarette smoke condensate (CSC). As negative control served BEAS-2B cells that were untreated or treated with CSC/DMSO for one month without the subsequent application of 5Aza/TSA. Experiment Overall Design: BEAS-2B Cells were treated for one month with CSC, DMSO, and left untreated. Subsequently half of the samples were treated with the demethylation agent. So that there were six different conditions with three biological replicates each. One sample had to be excluded because of low quality.

2009-08-15 | E-GEOD-14385 | biostudies-arrayexpress

Transcription profiling of human bronchial epithelial cells to low doses of cigarette smoke condensate - chronic exposure

Project description:BEAS-2B cells have been treated with low doses (20 ug/ml) of CSC for 4 months. As negative control BEAS-2B cells were treated with DMSO (the CSC solvent). Non-treated cells were cultivated in parallel. Experiment Overall Design: After each month total RNA was extracted from three replicates of CSC, DMSO and non-treated BEAS-2B cells and hybridized to Affymetrix GeneChips.

2009-08-09 | E-GEOD-14383 | biostudies-arrayexpress

Expression data from human lung epithelial cells after amorphous silica nanoparticle exposure for 40 passages

Project description:Amorphous silica nanoparticles induce malignant transformation and tumorigenesis of human lung epithelial cells. We used microarrays to detail the global programme of gene expression underlying the cellular malignant transformation induced by amorphous silica nanoparticles and identified distinct classes of up-regulated and down-regulated genes during this process. The human lung epithelial cells, Beas-2B were continuously exposed to 5 Î¼g/mL amorphous silica nanoparticles for 40 passages, and named as BeasSiNPs-P40 (shortly as P40-5 during the further microarray detection). Meanwhile, the passage-matched control Beas-2B cells, named as Beas-P40 (shortly as NC during the further microarray detection).

2016-06-01 | E-GEOD-82062 | biostudies-arrayexpress

Methylation arrays (MethylationEPIC) of human lung epithelial BEAS-2B cells exposed to reduced graphene oxide

Project description:Nanomaterials have lots of promising applications, and concern has risen about their impact to human health. Here, we have analyzed the genome-wide DNA methylation changes associated to the exposure to reduced graphene oxide (rGO) in human lung epithelial cells. Six conditions were assayed, with two technical replicates per condition (12 arrays in total): control, 1 and 10 µg/mL of rGO for 15 or 30 days of exposure.

2019-09-16 | E-MTAB-7719 | biostudies-arrayexpress

BEAS-2B cells: Control versus chemical exposure

Project description:Transcriptonal profiling of BEAS-2B cells: Control versus skin sensitizers; Control versus respiratory sensitizers; Control versus non-sensitizing irritants Replicates: 3 biological replicates; Exposure: 10 different chemicals versus solvent, 1 exposure concentration (IC20); Exposure time: 3 different exposure time points;

2013-12-01 | E-GEOD-44729 | biostudies-arrayexpress

Lung cell responses to ship diesel exhaust particles

Project description:Human BEAS-2B bronchial epithelial cells were exposed directly at the air-liquid interphase towards exhaust gas and particles of a ship engine. The goal was to compare the responses towards different fuel combustions. The engine run either on diesel fuel (DF) or on Heavy Fuel Oil (HFO). The lung cells were exposed 3 times to each combustion aerosol (DF or HFO). The duration of the exposure was 4h. The cells were seeded into transwell-inserts 24h before exposure. Within each exposure 3 transwell-inserts were exposed to the complete aerosol and 3 transwell-inserts were exposed to the filtered aerosol. Effects of the complete aerosol were referenced against the filtered aerosol to determine the effects of the aerosol particles.

2015-02-01 | E-GEOD-63962 | biostudies-arrayexpress

Anti-influenza activity of succinate

Project description:In the present study, we discovered an unexpected interplay between immunometabolism and antiviral immunity. Profiling of human bronchial epithelial BEAS-2B cells was performed using Agilent’s SurePrint G3 human gene expression microarray kit. A single-color design provided two types of comparison: (i) IAV-infected versus mock-infected cells, and (ii) succinate-treated infected cells versus mock-infected cells.

2022-02-17 | E-MTAB-10031 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data