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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of metabolically labelled RNA by 4-thiouracil from mouse tissues

ABSTRACT: To identify cell type-specific RNA without cell sorting, mice expressing UPRT in a specific cell type were exposed to 4-thiouracil to label RNA only in the UPRT-expressing cells. Total RNA was extracted from tissues and treated with iodoacetamide followed by RNA-seq library preparation. The reads generated from 4-thiouracil-containing transcripts should contain T to C mismatches as a result of 4-thiouracil incorporations and thus can be identified as the transcripts generated in the UPRT-expressing cells.

INSTRUMENT(S): Illumina HiSeq 1500

ORGANISM(S): Mus musculus

SUBMITTER: Wayo Matsushima

PROVIDER: E-MTAB-6353 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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